Research update: Watch the WWL-TV special report on West Nile Virus research from the Biochemistry laboratory at Southeastern (Drs. Doughty and Temple). Click: for RealPlayer: West Nile Virus; for MediaPlayer: West Nile Virus

Dr. Michael B. Doughty Associate Professor of Biochemistry Department of Chemistry and Physics Southeastern Louisiana University SLU Box 10878, Hammond, LA 70402 985-549-5123, Fax: 985-548-5126 email inquiries welcomed: mdoughty@selu.edu Office Hours

Undergraduate Research Opportunities: Positions now available on NIH funded research project Salaries range from $7.95 to $15.00 per hour, depending on experience, up to 20 hrs/ week during semester, 40 hrs/week otherwise. Requires: Enrollment in a Bachelor of Science (BS) program at Southeastern; Interest in a career in Biomedical Sciences; 3.0 GPA or above; all levels (2nd semester Freshmen to Senior) are eligible.. Inquiries: mdoughty@selu.edu
Research Interests Organic chemistry: synthesis of  nucleosides and nucleotides as enzyme inhibitors and structural probes. Biochemistry and Bio-organic chemistry: purification and analysis of enzymes and chemically modified enzymes using modern chromatography and analytical techniques; molecular cloning of viral DNA and RNA polymerases and reverse transcriptase. Bioassays (Biochemistry & Pharmacology): analysis of nucleotide inhibitors and structural probes against polymerases, particularly of E. coli DNA polymerase I, HIV reverse transcriptase, and more recently RNA polymerases of viral origin. Medicinal Chemistry: design improved inhibitors of viral, bacterial and cancer cell growth (termed antiviral, antibacterial, and anticancer agents) using modern structure-activity relationship (SAR) techniques. Molecular modeling: analysis of structures of organic molecules, particularly enzyme inhibitors, using molecular modeling and dynamics. Molecular cloning and expression: cloning (with Dr. Temple) of West Nile Virus RNA polymerase, and working on bacterial, human H1, and human H2 IMP dehydrogenases.	Professional Experience Teaching Experience & Awards Recent Publications Presentations PhD students mentored Undergraduate students Teaching Philosophy

PowerPoint Presentation: Research at SLU

Office and Teaching Schedule, Fall 2007*

Hours	M	T	W	TH	F
8:00 AM
8:30 AM
9:00 AM	OFFICE HOUR  PH 120A	OFFICE HOURS PH 120A	OFFICE HOUR  PH 120A	OFFICE HOURS PH 120A
9:30 AM
10:00 AM
10:30 AM
11:00 AM	CHEM 481 PH 211		CHEM 481 PH 211		CHEM 481 PH 211
11:30 AM
12:00 PM	CHEM 121 PH 211		CHEM 121 PH 211		CHEM 121 PH 211
12:30 PM
1:00 PM
1:30 PM
2:00 PM	CHEM 103-03 SBA 230 PH 223		CLAB 283 PH 128 PH 221
2:30 PM
3:00 PM
3:30 PM
4:00 PM
4:30 PM
5:00 PM

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

At all other times Dr. Doughty can usually be found either in his office (120A PH) or laboratory (120 PH) and welcomes visitors.

Teaching Experience and Awards 

Positions held:

• Associate Professor of Biochemistry, Department of Chemistry and Physics, August 2001-present, Southeastern Louisiana University, Hammond, LA

• Professor of Medicinal Chemistry, April 1998 to July 31, 2001, The University of Kansas, Lawrence, KS.

• Associate Professor of Medicinal Chemistry, April 1993 to April 1998, The University of Kansas, Lawrence, KS.

• Assistant Professor of Medicinal Chemistry, January 1987 to April 1993, The University of Kansas.

• NIH postdoctoral fellow, 1985 to 1987, The Rockefeller University, Laboratory of Bioorganic Chemistry and Biochemistry, Research Director: the late Professor E. Tom Kaiser.

• Rockefeller Postdoctoral Fellow, 1983 to 1985, The Rockefeller University, Laboratory of Bioorganic Chemistry and Biochemistry, Research Director: the late Professor E. Tom Kaiser.

• Graduate Research and Teaching Assistant, 1978 to 1982, Louisiana State University, Research Director: Professor G.E. Risinger.

Awards:

• University of Kansas H.O.P.E. (Honor for the Outstanding Progressive Educator) Award Finalist, selected by the Senior Class, 1995.

• Archie and Nancy Dykes Award for Outstanding Classroom Teaching, The University of Kansas, 1996.

• Kemper Fellowship for Teaching Excellence,  University of Kansas, 1998.  

Publications since 1990

• M. B. Doughty, S. S. Chu, D. W. Miller, K. Li and R. E. Tessel, Benextramine: a long-lasting neuropeptide Y receptor antagonist, European Journal of Pharmacology, 1990, 185, 113-114.

• M. B. Doughty, S. S. Chu, G. A. Misse and R. E. Tessel, Neuropeptide Y (NPY) functional group mimetics: Design, synthesis and characterization as NPY receptor antagonists, Bioorganic and Medicinal Chemistry Letters, 1992, 2, 1497-1502.

• M. B. Doughty, K. Li, L. Hu, S. S. Chu and R. E. Tessel, Benextramine-neuropeptide Y (NPY) binding site interactions: Characterization of ³H-NPY binding site heterogeneity in rat brain, Neuropeptides, 1992, 23, 169-180.

• M. B. Doughty, C. Chaurasia and K. Li, Benextramine-neuropeptide Y receptor interactions: Contribution of the benzylic moieties to ³H-NPY displacement activity, Journal of Medicinal Chemistry, 1993, 36, 272-279.

• M. B. Doughty and L. Hu, The contribution of helical potential to the in vitro receptor binding activity of a neuropeptide Y N-terminal deletion fragment, Biopolymers, 1993, 33, 1195-1206.

• G. A. Misse, S. S. Chu, A. Oke, M. B. Doughty and R. E. Tessel, Characterization of SC 3117, a novel Y₂ receptor antagonist, Society for Neuroscience, Abstracts, 1993, 19, 727.

• R. E. Tessel, D. W. Miller, G. A. Misse, X. Dong and M. B. Doughty, Characterization of vascular postsynaptic NPY receptor function and regulation.  1.  NPY-induced constriction in rat femoral artery rings is mediated by both Y₁ and Y₂ receptors: evidence from benextramine protection studies, Journal of Pharmacology and Experimental Therapeutics, 1993, 265, 1-6.

• R. E. Tessel, D. W. Miller, G. A. Misse, X. Dong and M. B. Doughty, Characterization of vascular postsynaptic NPY receptor function and regulation and differential sensitivity of Y₁ and Y₂ receptors to changes in extracellular calcium availability and prior in vitro peptide exposure, Neuropeptides, 1993, 25, 289-298.

• C. Chaurasia, G. Misse, R. Tessel and M. B. Doughty, Non-peptide peptidomimetic antagonists of the neuropeptide Y receptor: Benextramine analogs with selectivity for the peripheral Y₂ receptor, Journal of Medicinal Chemistry, 1994, 37, 2242-2248.

• L. Hu, P. Balse and M. B. Doughty, Neuropeptide Y N-terminal deletion fragments: Correlation between solution structure and receptor binding activity in rat brain cortex, J. Med. Chem., 1994, 37, 3622-3629.

• L. Hu and M. B. Doughty, Neuropeptide Y acylation chemistry in aqueous solution: Significance to synthesis of a peptide-based photoaffinity label, Journal of Protein Chemistry, 1994, 13, 135-140.

• M. J. McLeish, K. J. Nielsen, L. V. Najbar, J. D. Wade, F. Lin, M. B. Doughty and D. J. Craik, Conformation of a peptide corresponding to T4 lysozyme residues 59-81 by NMR and CD spectroscopy, Biochemistry, 1994, 33, 11174-11183.

• D. R. Benson, B. R. Hart, X. Zhu and M. B. Doughty, Design, synthesis and circular dichroism investigation of a a peptide-sandwiched mesoheme, J. Am. Chem. Soc., 1995, 117, 8502-8510.

• S. S. Chu, D. Shobe, D. Vander Velde and M. B. Doughty, Conformational properties of the proline region of porcine neuropeptide Y by circular dichroism and ¹H-NMR spectroscopy, Biopolymers, 1995, 35, 583-593.

• D. Flaherty, P. Balse, K. Li, B. M. Moore and M. B. Doughty, Synthesis of 2-azido-1,N⁶-etheno and 2-azido analogs of deoxyadenosine as nucleotide photoaffinity probes, Nucleosides and Nucleotides, 1995, 14, 65-76.

• J. W. Izard, M. B. Doughty and D. A. Kendall, The Physical and Conformational Properties of Synthetic Idealized Signal Sequences Parallel their Biological Function, Biochemistry, 1995, 34, 9904-9912.

• Doughty, M.B., Balse, P., and Tessel, R., Non-peptide mimics of neuropeptide Y: Analysis of benextramine structure-activity relationships at Y1 and Y2 receptors, in Peptides: Chemistry, Structure and Biology, P.T.P. Kaumaya and R.S. Hodges, Editors. 1996, Mayflower Scientific, Ltd.: West Midlands, England. p. 711-712.

• B. M. Moore, R. Jalluri and M. B. Doughty, DNA Polymerase Photoprobe 2-(4-Azidophenacyl)thio-dATP Identifies the Template Binding Region of the DNA Polymerase I Klenow Fragment, Biochemistry, 1996, 36, 11642-11651.

• B. M. Moore, K. Li and M. B. Doughty, Deoxyadenosine-based DNA polymerase photoprobes:  Design, synthesis and characterization as inhibitors of DNA polymerase I Klenow fragment, Biochemistry, 1996, 35, 11634-11641.

• Ke Li, Weiying Lin and Kar Chung, Bob M. Moore, and M. B. Doughty, Template-competitive inhibitors of HIV I reverse transcriptase: Design, synthesis and inhibition activity, 2002, Bioorganic and Medicinal Chemistry , 10, 507-515.

• Weiying Lin , Ke Li, and Michael B. Doughty, Characterization of a binding site for template-competitive inhibitors of HIV I reverse transcriptase using photolabeling derivatives, 2002, Bioorganic and Medicinal Chemistry, 10, 4131-4141.

• Weiying Lin, K. Li, Bob Moore II, and Michael B. Doughty, Conformational properties of template competitive HIV-1 reverse transcriptase inhibitors: Analysis of enzyme binding modes, 2003 , Nucleosides, Nucleotides, and Nucleic Acids, 22 (3), 283-297.

• Andreas Gille, Katharina Wenzel-Seifert, Michael B. Doughty, and Roland Seifert, GDP affinity and order state of the catalytic site are critical for function of xanthine nucleotide-selective Gas proteins, 2003, Journal of Biological Chemistry, 278, 7822-2828

• Andreas Gille, Gerald H. Lushington, Tung-Chung Mou, Michael B. Doughty, Roger A. Johnson, and Roland Seifert, Differential Inhibition of Adenylyl Cyclase Isoforms and Soluble Guanylyl Cylcase by Purine and Pyrimidine Nucleotides, 2004, Journal of Biological Chemistry, 279, 19955–19969.

• Michael B. Doughty*¹, Zac Aboudehen, Garland Anderson, Ke Li, Bob M.Moore, and Tina Poolson, Side-Chain Conformational Restriction in Template-Competitive Inhibitors of E. coli DNA Polymerase I Klenow Fragment: Synthesis, Structural Characterization, and Enzyme Inhibition Activity, Nucleosides, Nucleotides, and Nucleic Acids,  2004,  23, 1751 - 1765.

   

Presentations since 1990

• Abstracts

• R.E. Tessel, D.  Xu, D.W. Miller, and Michael B. Doughty, NPY(13-36) and [Leu-Pro]NPY constrict rat femoral artery rings, and protect NPY from benextramine blockade by acting at Y1 and Y2 receptors, Society for Neuroscience, Abstracts, 1991, 17, 976.

• Chandra Chaurasia, Ke Li, and Michael B.  Doughty, 2-Naphthylbenextramine: A prelude to the development of selective and potent non-peptide neuropeptide Y antagonists, Society for Neuroscience, Abstracts, 1991, 21, 799.

• Longqin Hu and Michael B. Doughty, The effect of deletion of the N-terminal residues of neuropeptide Y (NPY) on NPY receptor binding, Abstracts: 203rd American Chemical Society National Meeting, 1992, MEDI 85.

• Longqin Hu and Michael B. Doughty, The importance of the amphiphilic a-helix of neuropeptide Y (NPY) in structure formation and NPY receptor binding, Abstracts: 203rd American Chemical Society National Meeting, 1992, MEDI 86.

• G. A. Misse, S. S. Chu, A. Oke, M. B. Doughty and R. E. Tessel, Characterization of SC 3117, a novel Y₂ receptor antagonist, Society for Neuroscience, Abstracts, 1993, 19, 727.

• Richard E. Tessel, Michael B. Doughty and Gregory A. Misse, CC-2137 and PYY distinguish between peripheral and central Y₁ and peripheral and central Y₂ receptor in rat, Society for Neuroscience, Abstracts, 1994.

• Bob M. Moore and Michael B. Doughty, Photoaffinity Probes of DNA polymerase structure and function, Winter Conference for Bioorganic and Medicinal Chemistry, 1995.

• Ke Li, Bob M. Moore and Michael B. Doughty, Photoprobes of nucleic acid polymerase substrate binding sites, Winter Conference for Bioorganic and Medicinal Chemistry, 1995.

• Ke Li, Bob M. Moore and Michael B. Doughty, Photoaffinity probes of nucleic acid polymerase substrate binding sites, Joint meeting of American Society for Biochemistry and Molecular Biology and Division of Biological Chemistry of the American Chemical Society, 1995.

• D. R. Benson, B. R. Hart, X. Zhu, M. B. Doughty and F. D'Souza, Design and synthesis of a peptide-sandwiched mesoheme: Variable Heme Features in CD Spectra as a Function of Peptide Conformation, Metals in Biology Gordon Conference, Ventura, CA, 1995.

• Michael B. Doughty and Bob M. Moore. Photoaffinity Probes of DNA Polymerase Structure and Function, Joint meeting of American Society for Biochemistry and Molecular Biology and Division of Biological Chemistry of the American Chemical Society, 1995.

• Jennifer W. Izard, Michael B. Doughty and Debra A. Kendall. Physical Properties of Synthetic Idealized Signal Sequences Parallel their Biological Function, Joint meeting of American Society for Biochemistry and Molecular Biology and Division of Biological Chemistry of the American Chemical Society, 1995.

• Doughty, M.B., Balse, P., and Tessel, R., Non-peptide mimics of neuropeptide Y: Analysis of benextramine structure-activity relationships at Y1 and Y2 receptors, American Peptide Symposiumm, 1996.

• Preeti M. Balse, Richard E. Tessel, Murukambat N. Kumar and Michael B. Doughty.  Non-Peptide Mimics of Neuropeptide Y: A Structure-Activity Study of Benextramine Analogs.  American Chemical Society, March 1996.

• Ke Li and Michael B. Doughty. Nucleotide Photoprobes Define HIV-I Reverse Transcriptase Inhibitor Binding Sites,  American Chemical Society, March 1996.

• Invited Lectures

• “cAMP-Dependent Protein Kinase Peptide Substrate Interactions: A Pseudo Peptide Approach”, Department of Biochemistry, University of Kansas, 1988

• "Neuropeptide Y Structure and Function", State University of New York, Stony Brook.

• "Neuropeptide Y Structure and Antagonist Design", Parke-Davis Pharmaceutical Research, May 18, 1993.

• "The PP-fold in the Structure and Function of Neuropeptide Y and the Design of Non-peptide Antagonists", Synaptic Pharmaceutical Corporation, May 15, 1994.

• "Neuropeptide Y Structure and Function", State University of New York, Stony Brook, August 12, 1994.

• “DNA polymerase I Klenow Fragment Photoprobes: Identification of Substrate Binding Sites”, Department of Microbiology, University of Kansas, 1996
• Undergraduate Student Posters
  • Garland E. Anderson and Michael B. Doughty, "Triphosphate Inhibitors of Varied Length and Composition and Examination of Effects on HIV Reverse Transcriptase, 2004, Tenth Annual Student Conference for Research and Creative Arts, University of Houston Clear Lake.

   

Ph.D. Students Mentored

• Shao Song Chu, 1986-1991, PH.D. Thesis: "Neuropeptide Y Structure Activity: The Design of Functional Group Mimetics as Selective Antagonists".

• Diane Waltermire Flaherty, 1987-1990, M.S. Thesis: 2-Azido Analogs of dAMP as Photoaffinity Analogs of DNA Pol I Klenow Fragment".

• Longqin Hu, 1987-1992, Ph.D. Thesis: "Neuropeptide Y Analogues as Probes of a Bioactive Tertiary Structure", Irsay Dahle Award for Outstanding Graduate Student in Department of Medicinal Chemistry.

• Bob Moore, 1987-1995, Ph.D. Thesis: "Photoprobes of Nucleic Acid Polymerase Structure and Function", Graduate Honors Seminar, School of Pharmacy, 1995.

• Ke Li, 1991-1997, Ph.D. Thesis: Identification of HIV-1 Reverse Transcriptase substrate Binding Sites by a Selective Nucleotide Photoprobe: Implications for Inhibitor Design, Graduate Honors Seminar, School of Pharmacy, 1996.

• Pretti Balse, 1992-1998, Ph.D. Thesis: Neuropeptide Y Sructure and Function: Constrained Peptidomimetics and Non-peptide Mimics as Receptor Probes.

• Weiying Lin, 1996-2001, Ph.D. Thesis: Template-Competitive HIV-1 Reverse Transcriptase Photoprobes and Inhibitors: Characterization of the Inhibitor Binding Site via Photoaffinity Labeling

Undergraduate Students Mentored

At the University of Kansas